This initial round of financing from NSF will enable Akonni to measure the feasibility of developing a lab-on-a-film microarray device that can be stated in a reel-to-reel format. This indicates that, with tightening ballooning and budgets projections for diagnostic testing, another technical revolutions in molecular diagnostics must deliver significant reductions in per test cost. ‘Reel-to-reel assembly is certainly a method of high quantity manufacturing utilized predominantly for the assembly of lateral flow strips and flexible film electronics,’ said Dr. Christopher Cooney, Principal Investigator on the Director and grant of Engineering at Akonni Biosystems. ‘The advantage of this manufacturing strategy is that lab-on-a-film microarray production and assembly could be automated at high speeds, leading to ten to one hundred-fold cost savings in costs.’ If effective, lab-on-a-film manufacturing has the potential to create highly multiplexed microarray gadgets for just a few dollars.Bacterial identification was achieved by means of a industrial latex agglutination kit and the coagulase test. Antimicrobial-susceptibility assessment was performed by using a disk-diffusion technique13 for the following medications: cefoxitin, erythromycin, ciprofloxacin, gentamicin, tetracycline, rifampin, fusidic acid, and mupirocin. Extra antimicrobial susceptibilities to clindamycin, kanamycin, tobramycin, trimethoprim, and linezolid were dependant on method of an automated program . DNA Analysis and Sequencing DNA extracted from each MRSA isolate was prepared for sequencing with the use of a kit . Samples were pooled together and operate on a sequencer for paired-end 150-bp sequencing.